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Research

David R. Brigstock, Ph.D.


700 Childrens Dr
Columbus, OH 43205

Phone: (614) 722-2840

Email: brigstock.1@osu.edu

 

Current OSU Appointments

Professor, Pediatric Surgery

Professor, SBS-Biolog Chem & Pharmacology

 

Academic Advising

present Alpana Chaudhuri, Ohio State University.
1994 - present Randolph Cribbs,, United States Air Force.
1999 - present Marc Michalsky,, Nationwide Children's Hospital.
2003 - present Zhen-Yue Tong, Ohio State University.
 

Degrees

1988 B.S., Brunel University

1998 Ph.D., University of Cambridge

 

Editorial Activities

1991 Growth factors in the uterus: Steroidal regulation and biological actions
1994 Control of embryonic development by uterine growth factors and cytokines
1999 The connective tissue growth factor/cysteine-rich61/nephroblastoma overexpressed (CCN) family
1999 Purification and characterization of connective tissue growth factor (CTGF) using heparin-affinity chromatography. In: Aboul-Enein, H.Y (ed) "Analytical and Preparative Separation Methods of Biomacromolecules"
1999 Characterization and partial purification of steroidogenic factors from thymic epithelial cell conditioned medium. In: Aboul-Enein, H.Y (ed) "Analytical and Preparative Separation Methods of Biomacromolecules"
2000 Connective Tissue Growth Factor: What's in a name?
2002 Angiogenesis: The shared roles of CTGF and CYR61
2003 CTGF in hepatic fibrosis
2005 Editorial: CCN Workshop - Report on the Third International Workshop on the CCN Family of Genes
2005 CCN proteins in liver injury and disease. In “CCN Proteins: A New Family of Cell Growth and Differentiation Regulators"
2010 Inhibitors of connective tissue growth factor (CCN2)-mediated fibrogenesis: Underlying mechanisms and prospects for anti-fibrotic therapy
2012 Regulation of pancreatic function by connective tissue growth factor (CTGF,CCN2).
 

Honors

1986 - present New Investigator Award.
1996 - present Finalist. Society for the Study of Fertility.
2000 - present Invited Speaker. Gordon Conference on Reproductive Tract Biology.
2002 - present Invited Speaker & Session Chairman.
2002 - present Invited Speaker & Session Chairman. International Symposia on CCN Gene Family (1st 2000, 2nd 2002, 3rd 2004, 4th 2006).
2002 - present Fellow. Society of Biology.
 

Journal Articles

 

Inventions and Patents

Antibodies to heparin-binding growth factor polypeptides

Heparin-binding growth factor polypeptides

 

Presentations

Heparin-binding polypeptide growth factors. Wexner International Exchange Program Research Colloquium

A new aspect in the wound healing module - heparin-binding epidermal growth factor (HB-EGF). What role does heparin binding play?

Heparin-dependence of the interaction of heparin-binding EGF-like growth factor (HB-EGF) with the EGF receptor

Fibroblast growth factors: From bench to bedside

A 55,000-Mr mitogen in pig uterine flushings

A novel heparin-binding growth factor in porcine uterine luminal fluid

Heparin-binding growth factors in the uterus (Invited Speaker)

Heparin-binding epidermal growth factor (HB-EGF): A novel growth factor with potential intestinal cytoprotective effects

A novel heparin-binding growth factor in uterine luminal fluid

Heparin-binding growth factors in uterine secretions

Heparin-binding epidermal growth factor (HB-EGF) - New implications in wound healing

An Introduction to Growth Factors

Specific protein expression by a metastasizing clone of murine neuroblastoma

Novel forms of epidermal growth factor in uterine fluids

Human endometrial carcinoma cells secrete a heparin-binding mitogen that is similar or identical to platelet-derived growth factor

Novel secretory growth factors in the uterus of the pig

Uterine growth factors in embryo development and survival

Specific protein expression by a metastasizing clone of murine neuroblastoma

Human burn wound fluid contains heparin-binding EGF-like growth factor (HB-EGF) and platelet-derived growth factor (PDGF)

Epidermal growth factor in uterine fluids

Uterine growth factors in embryonic growth and development

Novel polypeptide growth factors in the uterus

Hyper-responsiveness of atherogenic vascular smooth muscle cells to members of the EGF family

Characterization of a polypeptide growth factor in human urine

Production and characterization of recombinant human HB-EGF in E. coli

Human burn wound fluid contains HB-EGF-like factors and PDGF-like factors

A novel heparin-binding growth factor in pig uterine secretions

Epidermal growth factor in uterine fluids

Heparin-binding EGF-like growth factor in the pig uterus

Human burn wound fluid contains two distinct heparin-binding growth factors

Urinary heparin-binding growth factor (uHBGF)

Maternal growth factors as regulators of embryonic development (Invited Speaker)

Heparin-binding growth factors in pig uterine fluids (Invited Speaker)

Comparative protein expression by C1300 murine neuroblastoma and its metastasizing clone, TBJ

Immunohistochemical detection of heparin-binding EGF-like growth factor (HB-EGF).

Human burn wound fluid contains heparin-binding EGF-like growth factor (HB-EGF)

Immunodetection of heparin-binding EGF-like growth factor in multiple epithelial tissues

Immunodetection of heparin-binding EGF-like growth factor in multiple epithelial tissues

Synthesis of heparin-binding EGF-like growth factor (HB-EGF) by endometrial tissues and its isolation from uterine secretory fluids

Expression of heparin-binding EGF-like growth factor (HB-EGF) in eukaryotic cells using a vaccinia virus (VV) vector

Antibody affinity purification of urinary heparin-binding growth factor

Immunohistochemical detection of HB-EGF in multiple epithelial tissues

Production of the precursor form of heparin-binding EGF-like growth factor

Production of heparin-binding EGF-like growth factor using a vaccinia virus expression system

Immunohistochemical survey of epithelial tissues and tumors for the presence of heparin-binding EGF-like growth factor (HB-EGF)

Connective tissue growth factor in the female reproductive tract

Structural organization of the gene for murine heparin-binding epidermal growth factor-like growth factor (HB-EGF)

Structural organization of the gene for murine heparin-binding epidermal growth factor-like growth factor (HB-EGF)

Uterine secretory fluids contain the developmentally-regulated neurotrophic factor, pleiotrophin

Production of HB-EGF at sites of thermal injury

A new growth factor in the pig uterus

Growth factors in prenatal life

Growth factors in prenatal life

CTGF: A new growth factor in the female reproductive tract (Invited Speaker)

Functional analysis of HB-EGF glycosylation

Discovery and isolation of a new polypeptide growth factor in the female reproductive tract

Functional expression of human HB-EGF cDNA in mouse L cells

Characterization and expression of recombinant CTGF proteins

An analysis of O-glycosylation in human heparin-binding EGF-like growth factor

Connective Tissue Growth Factor (Invited Speaker)

Bioactive CTGF fragments

Uterine connective tissue growth factor. (Invited Speaker)

Expression of CTGF and TGF-b1 in the pseudopregnant mouse uterus

CTGF in uterine function (Invited Speaker)

Establishment of a recombinant CTGF expression system in vitro that models CTGF processing in vivo: Structural and functional characterization of multiple mass CTGF proteins

Novel models of CTGF transgenesis in vivo: Recombinant adeno-associated viral (rAAV) mediated delivery of the CTGF gene

Localization of connective tissue growth factor in normal regenerating and denervated non-regenerating scarring limb stumps of adult newts

CTGF: Functions and Properties (Invited Speaker)

Connective tissue growth factor (CTGF) in liver fibrosis: Effects of CTGF on hepatic stellate cells and development of a rAAV- mediated delivery system for intra-hepatic CTGF gene delivery

Connective tissue growth factor is involved in cell cycle regulation in glioma cells

Connective tissue growth factor stimulates porcine fibroblast gene expression

Connective Tissue Growth Factor (Invited Speaker)

Heparan sulfate proteoglycans and low density lipoprotein receptor-related protein are involved in adhesion of rat activated hepatic stellate cells to CTGF

CTGF interactions with hepatic stellate cells

Connective tissue growth factor is involved in cell cycle regulation in glioma cells

Interaction of CTGF with hepatic stellate cells

Genes involved in fibrogenesis are transcriptionally up- or down-regulated following hepatic stellate cell (HSC) adhesion to connective tissue growth factor (CCN2)

Functional analysis of the thrombospondin Type 1 repeat (TSR) in human connective tissue growth factor

CCN2: interactions with cell surface heparan sulfate proteoglycans and intergins on fibrogenic cell types

Hepatic inflammation and fibrosis in transgenic mice that over-produce hepatocyte connective tissue growth factor

Decreased reproduction capacity in male connective tissue growth factor (CTGF/CCN2) transgenic mice

CCN2 in fibrosis (Invited Speaker)

Hepatic inflammation and fibrosis in transgenic mice that over-produce hepatocyte connective tissue growth factor (CTGF)

Mechanisms of CTGF action in the liver.

Hepatic inflammation and fibrosis in transgenic mice that over-produce hepatocyte connective tissue growth factor

The thrombospondin structural homology repeat in connective tissue growth factor is intrinsically active and stimulates hepatic stellate cell function

CTGF in hepatic fibrosis

CTGF structure, function and role in fibrosis (Invited Speaker)

Connective Tissue Growth Factor in Liver Injury - Lessons from Transgenic Mice

CTGF in liver disease

The thrombospondin structural homology repeat in connective tissue growth factor is intrinsically active and stimulates hepatic stellate cell function

CTGF in hepatic fibrosis

CTGF in hepatic fibrosis

CTGF siRNA delivered to fibrosing liver with a cyclic RGD peptide is anti-fibrotic. Gordon Research Conference on Samll Integrin-Binding Proteins

Hepatic over-expression of connective tissue growth factor abrogates fibrosis-resistance in the liver

Anti-fibrotic property of CTGF siRNA in the liver via targeted delivery to hepatic stellate cells.

Selective targeting of CTGF siRNA to activated stellate cells reduces CCl4-induced hepatic fibrosis in vivo

Liver Fibrosis: Problem Solved?

Connective Tissue Growth Factor: A new therapeutic target in liver fibrosis

The role of CTGF in pediatric acute lymphoblastic leukemia

Targeted CTGF gene therapy in experimental liver fibrosis

Therapy for hepatic fibrosis: Are we there yet?

Targeted delivery of connective tissue growth factor siRNA to activated hepatic stellate cells resolves experimental liver fibrosis in mice

A model of ethanol-induced pancreatic fibrosis in mice

Integrin α5β1 is a CTGF receptor on early primary culture hepatic stellate cells and interacts with fibronectin and heparan sulfate proteglycans to regulate CTGF function

Differential modulation of integrins gene expression by connective tissue growth factor in early and activated primary culture hepatic stellate cells

A model of ethanol-induced pancreatic fibrosis in mice

Connective tissue growth factor localizes to activated pancreatic stellate cells in a model of ethanol-induced pancreatic fibrosis

Resolution of experimental liver fibrosis in mice by targeted delivery of connective tissue growth factor siRNA

Ethanol-mediated connective tissue growth factor (CTGF) mRNA expression and promoter activity in human LX-2 hepatic stellate cells.

HB-EGF attenuates experimental liver fibrosis and reduces hepatic stellate cell activation

HB-EGF attenuates experimental liver fibrosis and reduces hepatic stellate cell activation

HB-EGF gene knockout exacerbates liver fibrosis in mice.

Ethanol-induced expression of connective tissue growth factor (CTGF) and promoter activity in human hepatic stellate cells

Connective tissue growth factor localizes to activated pancreatic stellate cells in a model of ethanol-induced pancreatic fibrosis

Connective tissue growth factor in liver fibrosis. Symposium on “Extracellular matrix proteins of the CCN family as therapeutic targets

CTGF is Up-regulated in a Mouse Model of Pancreatic Fibrosis.

Connective tissue growth factor localizes to activated pancreatic stellate cells in a model of ethanol-induced pancreatic fibrosis

Ethanol-induced expression of connective tissue growth factor (CTGF) and promoter activity in human hepatic stellate cells

Connective tissue growth factor (CCN2) modulates its cognate integrin receptors and promotes cell adhesion by interacting with fibronectin through integrin a5β1 in primary culture mouse hepatic stellate cells

Connective tissue growth factor (CCN2) modulates its cognate integrin receptors and promotes cell adhesion by interacting with fibronectin through integrin a5β1 in primary culture mouse hepatic stellate cells.

Cellular localization of connective tissue growth factor in pancreatic fibrosis in mice. In “Non-neoplastic Tissue and Organ Pathobiology

Anti-fibrotic targets that exploit the CTGF axis in hepatic stellate cells

Ethanol-dependent expression of connective tissue growth factor is associated with down-regulation of microRNA 214

Differential Responses of Human Nucleus Pulposus Cells with Varying Degenerative Levels to TGFβ3+Dex, CTGF, and Link-N. New Horizons In Intervertebral Disc Research

Immune responses in alcohol-induced pancreatic injury are associated with CCN2 expression and action in acinar cells

Increased susceptibility to liver fibrosis in mice expressing a connective tissue growth factor transgene in hepatocytes

The matricellular protein CCN5/WISP-2 is progressively expressed during hepatic stellate cell activation and limits production of the closely-related fibrogenic protein, connective tissue growth factor (CCN2/CTGF

Regulation of connective tissue growth factor (CTGF) expression in hepatic stellate cells by intercellular transfer of micro-RNA214: A paradigm for exosome-mediated control of fibrogenic signaling in the liver

Differential Responses of Human Nucleus Pulposus Cells with Varying Degenerative Levels of TGFβ3, CTGF, and Link-N

Connective tissue growth factor (CTGF) modulates macrophage recruitment during pancreatic alcohol injury in mice

The pro-fibrotic molecule, connective tissue growth factor (CTGF), is regulated by microRNA-214 (miR-214) in hepatic stellate cells

In vivo model to determine the role of connective tissue growth factor (CTGF) in childhood leukaemia

Connective tissue growth factor production by pancreatic acinar cells is associated with regulation of immune function in a mouse model of alcoholic chronic pancreatitis Abstract # 1298855

Up-regulation of connective tissue growth factor (CTGF) during liver fibrosis is associated with suppression of hepatic microRNA-214 (miR-214) expression: Identification of CTGF mRNA as a direct target of miR-214 in hepatic stellate cells.

Exosomal microRNA modulates pathways of liver fibrosis by regulating connective tissue growth factor (CTGF) expression in fibrogenic cells during chronic injury

Exosomal microRNA modulates pathways of liver fibrosis by regulating connective tissue growth factor (CTGF) expression in fibrogenic cells during chronic injury

New insights into mechanisms of CTGF-dependent hepatic fibrosis

Regulation of CCN2 fibrogenic pathways by exosomal microRNA

A novel biological role for connective tissue growth factor (CTGF): Modulation of inflammatory cell recruitment during alcohol-induced injury of the pancreas

MicroRNA-214-mediated suppression of connective tissue growth factor (CTGF) in hepatic stellate cells is associated with stimulation of miR-214 promoter activity by cellular or exosomal Twist-1.

Regulation of liver fibrosis by microRNA

174. Exosomal microRNA and CTGF in liver fibrosis

Functional expression of human HB-EGF cDNA in mouse L cells.

Characterization of low molecular mass forms of connective tissue growth factor (CTGF) that are biologically active and heparin-binding.

STRUCTURAL ORGANIZATION OF THE GENE FOR MURINE HEPARIN-BINDING EPIDERMAL GROWTH FACTOR-LIKE GROWTH-FACTOR (HB-EGF)

rAAV-mediated CTGF gene overexpression in skeletal muscle in vivo is associated with muscle fiber atrophy

Ac-SDKP reverses cardiac fibrosis in rats with renovascular hypertension

Production of a C-terminal fragment of CTGF in E. coli that mimics activity of the intact CTGF molecule

RAT THYMIC EPITHELIAL-CELL CULTURE CONDITIONED MEDIUM (TCM) CONTAINS 2 DISTINCT STEROIDOGENIC FACTORS

HEPARIN-BINDING EGF-LIKE GROWTH-FACTOR IS A CONSTITUENT OF PIG UTERINE LUMINAL FLUSHINGS

Immunohistochemical detection of connective tissue growth factor during phases of urodele limb regeneration

Function of Ctgf in islet development and beta cell proliferation

Localization of uterine connective tissue growth factor during early pregnancy in the mouse.

Immunohistochemical localization of connective tissue growth factor in human endometrium and decidua.

Immunohistochemical detection of connective tissue growth factor during phases of urodele limb regeneration

Biological characterization of a new heparin-binding growth factor in pig uterine luminal flushings.

Production and characterization of a recombinant high molecular weight form of human heparin-binding EGF-like growth factor (HB-EGF).

Identification and characterization of CTGF as a factor involved in islet development and beta cell expansion.

Immunohistochemical localization of Heparin-binding EGF-like growth factor in diseased skin

Expression of connective tissue growth factor in mouse uteri and early stage embryos.

Connective tissue growth factor (CTGF) induces adhesion of rat activated hepatic stellate cells via integrin alpha v beta 3 and heparan sulfate proteoglycans through its carboxyl-terminal domain.

Immunohistochemical localization of heparin-binding EGF-like growth factor (HB-EGF) in epithelial tissues.

 

Professional Activities

0 Manuscript Reviewer. Oncology.
0 Grant Reviewer. NIH Special Emphasis Panel ZRR1 BT-B for Exploratory Centers (P20).
0 Grant Reviewer. German Research Foundation (Deutsche Forschungsgemeinschaft) 2012.
0 Manuscript Reviewer. Molecular Biology of the Cell.
0 Manuscript Reviewer. Clinical Chemistry.
0 Manuscript Reviewer. Cell Communication and Signaling.
0 Manuscript Reviewer. Hepatology.
0 Manuscript Reviewer. Reproduction.
0 Manuscript Reviewer. Lancet.
0 Manuscript Reviewer. Atherosclerosis; Arthritis Research & Therapy.
0 Manuscript Reviewer. J Cell Biochem.
0 Manuscript Reviewer. Biochemistry and Cell Biology.
0 Manuscript Reviewer. Biology of Reproduction.
0 Grant Reviewer. The Wellcome Trust, UK.
0 Manuscript Reviewer. Am J. Pathology.
0 Manuscript Reviewer. J Bone Mineral Res.
0 Manuscript Reviewer. Bone.
0 Manuscript Reviewer. J. Cell Physiol.
0 Grant Reviewer. The Scottish Hospital Endowments Research Trust, Scotland UK.
0 Grant Reviewer. USDA National Research Initiative Competitive Grants Program.
0 Manuscript Reviewer. Diabetologia.
0 Manuscript Reviewer. Oncogene.
0 Manuscript Reviewer. Biochemistry.
0 Manuscript Reviewer. Growth Factors.
0 Manuscript Reviewer. J. Clin..
0 Manuscript Reviewer. J. Hepatology.
0 Grant Reviewer. USDA National Research Initiative Competitive Grants Program.
0 Manuscript Reviewer. J. Endocrinol.
0 Manuscript Reviewer. Gynecologic Oncology.
0 Manuscript Reviewer. J Cell Science.
0 Manuscript Reviewer. Reproduction, Fertility & Development.
0 Manuscript Reviewer. Molecular and Cellular Biology.
0 Visiting Professor. Tongi Medical Center and Chengdu Hospital, China.
0 Visiting Professor. Tongi Medical Center and Guangzhou Children’s Hospital, China.
0 Member. International Society for Extracellular Vesicles.
0 Member. Scientific Board, International CCN Society.
0 Manuscript Reviewer. J. Reprod Fertil.
0 Manuscript Reviewer. Molecular Endocrinology.
0 Grant Reviewer. German Research Foundation (Deutsche Forschungsgemeinschaft) 2011.
0 Grant Reviewer. NIH Special Emphasis Panel/Scientific Review Group 2013/01 ZRG1 DKUS-C (04)M.
0 Grant Reviewer. Council for the Earth and Life Sciences.
0 Manuscript Reviewer. Endocrinol. Metab.
0 Grant Reviewer. USDA National Research Initiative Competitive Grants Program in Animal Growth.
2004 Senior Editor. Journal of Endocrinology.
2007 Member of Editorial Board. Journal of Cell Communication and Signaling.
1986 - 1988 Member of Editorial Board. Biologist.
1989 - 1991 Board Member. Charlesbank Apartments Tenants Association.
1990 - 1991 President. Charlesbank Apartments Tenants Association.
2002 - 2004 Member of Editorial Board. Journal of Endocrinology.
2004 - 2006 Senior Editor. Journal of Endocrinology.
2003 - 2007 Member of Editorial Board. Cell Communication and Signaling.
2007 - 2008 Soccer Coach. Dublin Soccer League.
0 - 2010 Member. Society of University Surgeons (elected).
2005 - 2010 Board Member. Wedgewood Hills Homeowners Association.
2008 - 2010 Vice Chair. International CCN Society.
2006 - 2012 US Editor. Journal of Endocrinology.
2006 - 2012 US Editor. Journal of Endocrinology.